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. Native dimeric GR eluted at a volume corresponding to an apparent molecular weight of ~85 kDa in the calibrated column, indicating a fairly compact dimer structure
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. Since no crystal structure of ACYI is available, all four of the components (A and S, I, II, and III) of Trp fluorescence defined previously (33,34) were included in the curve-fitting process
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. Protein was extensively dialyzed against 2 L of the working buffer (5 mM HEPES buffer containing 10 mM NaCl and 2 mM β-mercaptoethanol, pH 7) at 278 K
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. INTRODUCTIONCalmodulin (CaM) is a Ca^sup 2+^ binding protein, present in all eukaryotic cells
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. 6 shows essentially the same linear dependence for k^sub ex^ values as when Eqs
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. Structure of calmodulin relined at 2
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. It also recognizes intermediate cases in which diffusional searches occur simultaneously in high- and low-dimensional spaces, such as a partially-stabilized two-dimensional gutter in the three-helix case, and provides a structure for introducing tailored, rather than indiscriminate, nonnative interactions
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. One of the molecules distributed on Chattonella was determined to be glycoprotein (Nagasaki et al
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. 0 (0/5)AcknowledgmentsWe would like to thank the following sea farming centers and universities for providing the fish eggs used in this study: Fukui Prefectural Sea Farming Center; Kyoto Prefectural Sea Farming Center; Faculty of Agriculture, Kyushu University; Osaka Prefectural Fisheries Station; Sea Farming Center of the Japan Sea-Farming Association; Fisheries Laboratory of Kinki University
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.MATERIALS AND METHODSSample preparationSEF2-1mp mimics the DNA- and CaM-binding region of the bHLH transcription factor SEF2-1
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