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. Deterministic pressure dissociation and unfolding of triose phosphate isomerase: persistent heterogeneity of a protein dimer
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. As a consequence the corresponding emission spectra that were measured during unfolding appear completely different (Fig
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. Before comparing the behavior of individual proteins it is convenient to divide the group into two classes: one (β-gal, α-amylase, and ALD), which binds ANS essentially only in the frozen state, and the rest, which bind a fair amount of ANS also in liquid solution (4,5,28)
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. That the peptide remained dimeric was also confirmed by SDS-PAGE
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. 1, close to the isoelectric point, the osmotic pressure was lower at the lower NaCl concentrations (0 mM, 100 mM) and was fitted by an adhesive hard sphere model
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. Ring**Institute of Food Research, Norwich Research Park, Norwich NR4 7UA, United Kingdom; and [dagger] Department of Food Processing, Tallinn Technical University, Tallinn 19086, EstoniaSubmitted April 8, 2005, and accepted for publication May 25, 2005
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. Data analysisConversion of the apparent K(d) obtained on membrane surfaces to the K(d) that would be obtained if the proteins were freely diffusing has been previously treated by assuming that the proteins are confined to interact within a reduced surface volume (ν) that is equal to the surface area of the membrane multiplied by the distance from the membrane surface into the aqueous phase where the proteins interact, which is taken to be 50 nm
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