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. The phase diagram was constructed by the FSD IR intensity at 1637 cm^sup -1^ versus that at 1628 cm^sup -1^ (Fig
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. Simulated data agree with theoretical predictions and the agreement is quantified in tenus of residuals between the theoretical distribution and those from simulations
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. that the large amphipathic surface in the dimer interface could be exposed to solvent upon monomer formation; furthermore, there is a network of aspartic residues involved in salt bridges at the dimeric interface (Fig
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. This value (311 ± 5 K, at 4 µM protein concentration, 315 ± 9 K, at 6 µM protein concentration) was similar, within the experimental uncertainty, to that obtained in the intact EI^sup ec^ (4), where two tryptophan residues are located at the C-terminal region of the protein
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. The transitions measured by CD became broader as the concentration increased, and although a small increase was observed when the concentration was raised (3 µM yielded T^sub m^ = 328
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. In contrast all thermal unfolded states (with the exception of those obtained from PPA) exhibit λ^sub max^ values around 340 nm, which correspond to much smaller red shifts upon unfolding
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. Presumably, the wetting force represents the driving force toward the wetting as well as good bonding
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. 33 K/min is sigmoidal and reaches a plateau at high temperatures, suggesting that the unfolding transition is complete at 99°C, yet the light-scattering curve recorded in the same experiment does not reach a plateau, and the signal continues to increase upon cooling from 99°C (Fig
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. METHODSMaterialsBovine insulin (from Sigma Aldrich, Steinheim, Germany) was used without further purification
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. Table 4Results of analyses of covariance testing for differences in slopesand elevations of annual absolute fecundity-age relation and annualrelative fecundity-age relation
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. The rate constants were expressed in units of an experimental reference time, the average burst duration, as discussed later
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. The PTS involves a cascade of phosphoryl-transfer steps from phosphoenolpyruvate (PEP) via phosphointermediates of the general cytosolic non-sugar-specific phosphotransferases enzyme I (EI) and the histidine-phosphocarrier (HPr) to sugar-specific enzyme II permeases
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.Determination of the rotational correlation timesA reliable estimate of the overall rotation correlation times is very important for the further analysis of the relaxation data
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. Hence, for any reasonable value of Δ^sup ex^ (up to 6 ppm), these residues are in fast exchange (k^sup -1^^sub ex^ [much greater than] Δω^sub I^)
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.26 BL; mean BD/BL=0
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