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First, we present a detailed description of the methods used in our work
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This is shown in Fig
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There is, however, a finite probability of finding small cavities (aA summary of the difference between correlated fluctuations in the EV limit and uncorrelated fluctuations for a Flory-like freely rotating chain is shown in Fig
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Proteins in frozen solutions: evidence of ice-induced partial unfolding
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This is reflected by the rapid increase in number of fibrils and the increasing complexity of fibrillar topology (Fig
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T^sub g^ and initial modulus were determined by dynamic mechanical analysis (DMA, Seiko SII DMS 210)
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The cleavage reaction was initiated by addition of substrate strands (500 pM) to the respective preformed ribozyme complex (100 nM) at room temperature and reaction progress was monitored by gel analysis of aliquots quenched with formamide at different time points
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