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. are in general agreement with each other and reinforce Tanford's hypothesis (3) that highly denatured proteins behave like chains in a good solvent
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. Changes in the CD signal at 208 nm (where the parallel polarized π-π* transition occurs) are the result of a flexibility of the structure (46)
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. 0) and in all cases, the intensity of the fluorescence at 480 nm was very high at low temperatures (between 288 and 293 K)
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. Identification of the N-terminal domain of enzyme I of the Excherichia coli phosphoenolpyruvate:sugar phosphotransferase system produced by proteolytic digestion
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. Apoptotic cells are increased in several chronic inflammatory lung diseases, including cystic fibrosis (CF), non-CF bronchiectasis, COPD, and asthma
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. Henson; personal communication; March 2006)
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. 4 ms were applied in a point resolved spectroscopy sequence (PRESS) (26), along with spoiling gradients of 1 ms and 100 mT/m between pulses, which led to a minimum echo time of 6
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. Large-amplitude thermal fluctuations, requiring adequate protein hydration, have been shown to be mandatory for the function of bacteriorhodopsin, the light-driven proton pump in the purple membrane of Halobacterium salinarum (7)
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. Although the dications studied showed no difference in their affinity for coil DNA, their compaction activities differed by 100-fold
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. The pressure cell was inserted into the optical module of the fluorometer allowing for fluorescence measurements under pressure
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. This suggests that the protein refolding detected by CD between 32 and 24°C upon relatively rapid cooling (Fig
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. These may or may not be identified as intermediate states, depending on their occupancy lifetime
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. was supported by a fellowship from Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET)
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. Our data on T^sub g^ of the IPP polymers (9 to 13°C) are consistent with reference 3, which reported that the T^sub g^ of IPP polymer ranges from about -30 to +20°C and depends on tacticity and thermal history
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. 023-55,344 -4,279Effect of Type 2 RPNSAIDsAverage monthly effect -38
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. Moreover, resolution-enhancing techniques, such as second derivative and 2D IR, have been shown to be sensitive tools to monitor the sequential events in protein conformational changes by FTIR (27,28,30,38-42)
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