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No variations in the T^sub m^ were observed among the three scan rales, and thus, the criterion for microreversibility was satisfied 20 80 calculator loan .
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MethodsSamples for fluorescent microscopy were illuminated with 365 nm ultraviolet light, and fluorescence images of DNA molecules were observed using a Zeiss Axiovert 135 TV microscope equipped with a 100× oil-immersed lens and recorded on S-VHS videotape through a Hamamatsu SIT TV camera 20 80 calculator loan .
Gα^sub s^ was purified from SP9 cells by the same methods used for Gα^sub q^ purification (see Runnels et al 20 80 calculator loan .
, K^sub p^ ~ 70 as opposed to 10 µM; Philip and Scarlata, 2004) 20 80 calculator loan .
Scarlata, unpublished), allowing us to measure the apparent K^sub d^ at a given membrane concentration (Fig 20 80 calculator loan .
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