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An additional experiment in which the incubation time of the protein in the presence of 3 M GdnHCl was increased from 90 min to 24 h showed that the small dimer fraction observed in Fig
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Finally, given the role of GR in the maintenance of cellular redox state, preservation of a stable enzymatic capacity is likely to be very important for cell survival (under stress situations, in particular)
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As a zinc-dependent enzyme, ACYI is quite stable against denaturation induced by heat stress
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A receptor for phosphatidylserine-specific clearance of apoptotic cells
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0Bi solder exhibited less wetting force, i
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The 400-MHz data were analyzed with ^sup 1^H and ^sup 15^N diameters of 6
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, 2003a), show that the heat- and denaturant-induced transitions in discoidal lipoproteins are kinetically controlled reactions that involve protein unfolding, dissociation, and lipoprotein fusion
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. After the buffer baseline subtraction, the data were normalized to protein concentration and expressed as molar residue duplicity, [Θ], No changes in [Θ] were detected with changes in the protein concentration in the range explored (5-600 µg/ml)
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. Protein unfolding was triggered by an increase in guanidinium hydrochloride (Gdm HCl) concentration from 0 M to 1-6 M
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.The prolonged 10-min incubation at 60°C promotes further elongation of the fibers even up to 2
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.In addition to the changes directly related to the intermolecular contact, CD4 binding also induces conformational changes away from the binding interface that are crucial for the subsequent events of viral entry into the host cell
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.0]= age at zero length; and [l
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.Manuscript approved for publication 30 March 2004 by the Scientific Editor
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