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Intrinsic fluorescence spectroscopy The samples for the fluorescence measurements were prepared by dissolved ACYI in 30 mM Tris-HCl (pH 7
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(11), we obtained a scaling exponent of v = 0
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For each of the sequences shown in Table 1
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This feature is shared with Gaussian chains (39), although clear differences exist in the magnitude of and correlation between conformational fluctuations
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Bioactive properties of milk proteins with particular focus on anticariogenesis, J
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N-glycosylation of human acetylcholinesterase: effects on activity, stability and biosynthesis
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020 [Angstrom], and the ^sup 15^N CSA value to -170 ppm
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This will arrest diffusion at the protein globule length scale
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5-30 min): formation of soluble oligomers; 3), second aggregation phase (30 min-20 h): generation of larger aggregates including spherical aggregates and short protofibrils; and 4), maturation phase (>20 h): generation of more extended fibrils
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Fluorescent probes for polymer free-volume
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, Fändrich et al
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Im7 folding mechanism: misfolding on a path to the native state
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. Finally, the solution was dialyzed against TBS (Tris buffer (pH 7
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. Hydrodynamic calculations can provide some indication as to the structural assignment of the internal motions identified from NMR relaxation experiments (see Supplementary Material)
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. These points and the liquid-glass transition boundary have been added to the nonequilibrium phase diagram (Fig
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. ANS bound to native TTR showed a major component with a lifetime of 16
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., 1998) and to elucidate the global geometry of the hairpin ribozyme in docked and extended states (Murchie et al
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. The ribozyme system is sampled at 40 times per measurement interval to simulate the effects of the threshold of 40 photons we used for the FRET analysis
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. In striking contrast, neither the 2WJ nor the 3WJ ribozymes can populate such a high-FRET state in the absence of loop A
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. Since the folding observed in the SL 4WJ ribozyme construct occurs in the absence of loop-loop interactions, the conformational change must be associated with the 4WJ helical junction element itself
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. Yaeger PC, Masi TL, Buck de Ortiz JL, et al
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