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With respect to corresponding CD spectra we observed for both types of samples (i
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4-angstrom structure of calmodulin-peptide complex
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In this method, the fluorescence of donor and acceptor are simultaneously recorded using avalanche-photodiodes as individual molecules traverse the excitation volume in a confocal microscope, and the FRET efficiencies from many such single-molecule observations are plotted in the form of a histogram
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