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CD measurements suggested that unfolding of GR was complete at 7 M GdnHCl, a result that was consistent with the iodide quenching data
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4 strongly supported the conclusion that pretransitional conformation changes occurred at temperatures where the enzyme retained high activity (see Fig
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Structure and stability of EI^sup SC^ in the presence of the effectors All the experiments in the presence of PEP and Mg^sup 2+^ were carried out at pH 7
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The metastability is with respect to solid-liquid coexistence, that is, the solutions are supersaturated
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0 the osmotic pressure is independent of ionic strength and increases monotonically with protein mass fraction
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In practice, the large difference in anisotropy decay between monomeric forms lead to a broad baseline with the contribution of the smaller monomeric forms contributing as a decay curve superimposed onto a baseline
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The molecular details of how PLCβ interacts with Gβγ is unknown
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This suggests that the aggregation process is essentially completed after 1 h at 70°C
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Protein folding and misfolding
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These may or may not be identified as intermediate states, depending on their occupancy lifetime
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When comparing the melting rates of the three polymers, the melting temperatures should be considered
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Percentage of all matureAge group Absolute females represented(yr)fecundity (1) by each age group6-8 364,183
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After 48 hours the cells had formed pellets, which were randomly subdivided into two groups
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