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. These features (namely, the absence of tertiary structure, the lack of a sigmoidal behavior in thermal heating, solvent-exposure of hydrophobic residues, and a large amount of secondary structure) are characteristic of the so-called molten-globule species (47,48)
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. Differential effects of apoptotic versus lysed cells on macrophage production of cytokines: role of proteases
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. MSDs increase continuously, but display a dynamical transition at ~200 K
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. The bottle was then placed in a homemade optical pressure cell based on the design of Paladini and Weber (1981)
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. 2) than those with apoA-1 (data not shown)
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. Possibly the greatest impact on our health will be the role these peptides play in maintaining normal blood pressure
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. The T^sub g^ of the Colorocs toner was reported to be 58°C [19]
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. Ann Emerg Med 1995; 26: 187-94
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. One bird, a Northern Cardinal, was represented by the ramphotheca from a lower mandible as well as by small body feathers
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. Usually, glycans do not generate permanent secondary structure but trigger local eonformational alterations close to the glycosylation site, particularly by inducing a β-turn at the site (13)
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. Are proteins well packed? Biophys
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.0; and sodium phosphate, pH 11
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. In EI^sup sc^, a single peak was observed in CD and DSC experiments, but in the pH range where the thermal transitions could be followed (pH 7-9), two different peaks were not observed; further, the T^sub m^ of the sole transition observed was pH-indcpendent between pH 7 and 9 (Fig
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. A similar difference in emission peak maxima between thermal and GndHCl-induced unfolding was also observed for other proteins (see for example (10,21))
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. Therefore, a purely diffusion-controlled on-rate k^sub on^ of 10 s^sup -1^ M^sup -1^ would put a lower limit on the CaM:SEF2-1mp affinity, k^sub off^/k^sub on^ > 10^sup -6^
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. The C^sub p^(T) data were recorded from degassed protein and/or buffer solutions upon repetitive heating from 5 to 100°C at a rate 90 K/h, followed by cooling and incubation at 24°C
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.10 This is demonstrated by the production of type-I collagen as opposed to type-II collagen which is characteristic of hyaline cartilage
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