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. In contrast, the recombinant protein unfolds via an intermediate
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. This value (311 ± 5 K, at 4 µM protein concentration, 315 ± 9 K, at 6 µM protein concentration) was similar, within the experimental uncertainty, to that obtained in the intact EI^sup ec^ (4), where two tryptophan residues are located at the C-terminal region of the protein
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. To enable conformational characterization of protein isolated from biological specimens such as samples from patients, noncovalent recognition using extrinsic dyes is necessary
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. Specifically, focusing on the prefibrillar amorphous aggregates occurring 30 s after elevating temperature to the nucleation-enhancing 60°C, revealed the tendency of the globule-shaped oligomers to queue and assembly into elongated forms
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.Monoclonal antibodies were developed according to the methods of Kohler and Milstcin (1975), Garfre and Milstein (1981), and Hiroishi et al
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. However, because we have compared results across a series of molecules where the RNA sequence (and secondary structure) in the vicinity of the dyes has been held constant, the differences between the characteristics we observe are related primarily to tertiary structural differences between the constructs
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.23 DNA from calf thymus (Sigma) was used to prepare a standard curve
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