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The FRET efficiency histogram of the GnI variant also exhibits two distinct subpopulations, with mean E values that are similar to the wild-type, but the amplitude of the high FRET peak is substantially reduced relative to the low FRET peak (Fig account into loan payday savings .
Consistent with this hypothesis, previous trFRET studies have shown that the 4WJ stabilizes the tertiary structure of the hairpin ribozyme by reducing the entropie penalty for docking as compared with ribozyme constructs containing either a 2WJ or 3WJ (Klostermeier and Millar, 2000) account into loan payday savings .
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