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Apomyoglobin is one of the proteins most extensively studied in the protein folding research
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For large x, entropy opposes stretching of the chain beyond its average value of R^sub e^
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Buffer contributions were subtracted, and the resulting spectra were used for analysis
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This investigation aims to complement that initial study by addressing the following issues: i), establish whether the perturbation of protein structure reported for azurin is general to other proteins; ii), inquire on potential correlations between the susceptibility to the ice-induced perturbation and specific features of the macromolecule such as thermodynamic stability, internal flexibility, internal cavities/hydration, and covalent links between strands; and finally iii), test for a specific vulnerability of multimeric proteins to freeze damage that may be linked to the disruption of quaternary structure
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EXPERIMENTAL PROCEDUREMeasurement of Contact Angle and Wetting ForceThe wettability test was performed using the solderability tester MENISCO ST 50 with computer control and data acquisition functions
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The four hinge regions create a flexible CaM dimer that can easily slide along or wobble over the peptide sequence allowing it to interact with the different binding sites in the interior of the CaM dimer
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Intramolecular transfer of excitation from tryptophan to 1-dimethylaminonaphthalene-5-sulfonamide in a series of model compounds
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Further support for this hypothesis comes from a large reduction in the apparent T^sub m^ and faster thermal unfolding observed upon disulfide reduction in apoA-2:DMPC disks (data not shown), which is consistent with the report that apoA-2 on the disks is less stable in monomeric than in dimeric form (Lund-Katz et al
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Manuscript submitted 20 November 2003 to the Scientific Editor's Office
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This indicates that the intermolecular interactions between gp120 and CD4, although decreasing the intraresidue entropy of residues in direct contact, can induce intraresidue entropy changes at the interdomain interface within the gp120 monomer and at the putative trimeric gp120 interface
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