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. The yield of the protein was 5-8 mg per liter of culture
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. We carried out measurements at different protein concentrations either by CD and fluorescence in the concentration range 4-8 µM at pH 7
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. Binding of the catabolite repressor protein to its DNA target is regulated by phopshorylation of its co-represser HPr
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. 4 A), up to sixfold, whereas the onset of binding occurs at either smaller or higher ANS concentrations, indicating larger and smaller binding affinities, respectively
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. In the analysis of the CaM data we used an axially symmetric diffusion model to calculate the relaxation parameters
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. 1 as measured by Grönwall (41) is plotted in Fig
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. There are four known families of PLCβ enzymes and all are activated by Gα^sub q^-subunits (for review, see Rebecchi and Pentyala, 2000; Rhee, 2001)
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. Since commercial toners generally contain less than 5 wt% colorant, we used 4 wt% pigment to produce the IPP and EVA toners
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. AcknowledgmentsThis study resulted from cooperative efforts of the USGS Biological Survey Unit and the Department of Zoology, National Museum of Natural History
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