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. Taken together, these results suggest the existence of two molten-globule-like intermediate species (one dimeric and one monomeric) in the unfolding of GR
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. 5 ns, S^sup 2^^sub s^ = 0
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. The shortest incubation at 60°C (or 70°C) applied to insulin samples was only 30 s; yet as Fig
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. was supported by a fellowship from Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET)
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. The ribozyme system is sampled at 40 times per measurement interval to simulate the effects of the threshold of 40 photons we used for the FRET analysis
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. For comparison, we also overlay histograms for twofold slower and faster rate constants (dashed lines), with the equilibrium constant maintained at 3
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. Thermal melts done with both rSBA and gSBA were found to he irreversible in nature, and visible aggregates were noticed at the end of the scans in either case
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