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The two positive peaks located at 1618 and 1682 cm^sup -1^ confirmed the appearance of the aggregates observed in Fig
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With each mutant azurin, a sizable fraction of binding sites exhibits affinities in the 1-2 mm range, corresponding to a dissociation constant 3-4 times lower than that of WT
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. Based on a two-component mixture theory, we obtained the following hydration numbers per protein molecule: 590 +/- 65 for N, 630 +/- 73 for A, and 1110 +/- 67 for U^sub A^
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. Whenever the size of a probed object permitted, 20 subsequent measurements of an individual feature were carried out and averaged
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. MOHLENBROCK is professor emeritus of plant biology at Southern Illinois University in Carbondale
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