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Thanks to an unusually blue shifted Trp fluorescence spectrum of native azurin, a measure of the protein fraction remaining native after freezing-thawing can be obtained directly from the residual fluorescence intensity at 300 nm (λ^sub ex^ = 292 nm), a wavelength where denatured-aggregated proteins were found to contribute little to the overall intensity
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CONCLUSION In vivo localized MR spectra were recorded from subcellular regions within X
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In a first experiment, we compared MSDs of native HuBChE and soman-inhibited HuBChE over a large temperature range (20 to ~349 K), passing the denaturation temperature of native HuBChE
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Proton chemical shifts were calibrated using the internal standard DSS (0
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In conclusion, we have shown that insulin amyloidogenesis in vitro induced by the en masse destabilization of native dimers proceeds through a multipathway assembling scheme, which employs-on the one hand-the hierarchical intertwisting reported earlier; and on the other hand, employs lateral interactions of single particles and fibrils, leading to larger domains of features aligned in parallel (Fig
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