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© 2003 by the Biophysical Society0006-3495/03/11/3255/07 $2
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In brief, the intrinsic Trp fluorescence emission spectra were measured using a Hitachi F-2500 spectrofluoromeler (Tokyo, Japan) using 1-cm path-length cuvettes with an excitation wavelength of 295 nm
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These results would make sense if denatured-state topologies were equivalent and invariant with sequence
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The use of circular dichroism in the investigation of protein structure and function
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CONCLUSION It has been demonstrated in this study that the λ^sub max^-values of tryptophan fluorescence emission are meaningful parameter to characterize properties of the unfolded state
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Drug delivery in biotechnology: present and future
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The contribution of vibrational entropy to molecular association
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Thus, in addition to the usual picosecond-timescale motions of varying contributions, a small amplitude nanosecond-timescale motion is present for all CaM residues in the CaM:SEF2-1mp complex
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Self-diffusion and cooperative diffusion of globular proteins in solution
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Polyethylene glycols with molecular weights of 3000 and 10000 were purchased from Merck (Hohenbrunn, Germany)
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We monitored membrane binding by FRET from PLCβ tryptophan side chains to DNS-PE incorporated into POPC/POPS (2:1) LUVs
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Symptoms of hepatotoxicity, such as nausea, vomiting, and abdominal pain, may not appear until more than 24 hours post ingestion, but liver damage--and even death--may occur if the antidote n-acetylcysteine (or NAC) is not administered within eight hours after ingestion
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** SOGS score [greater than or equal to] 5
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