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The equilibrium unfolding of GR by GdnHCl was monitored by measuring fluorescence emission spectra at various denaturant concentrations
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Variability in quaternary association of proteins with the same tertiary fold: a case study and rationalization involving legume lectins
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We carried out measurements at different protein concentrations either by CD and fluorescence in the concentration range 4-8 µM at pH 7
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Structural and dynamical features contributing to thermostability in alpha-amylases
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Recently, a study conducted in our laboratory investigated the possibility to unveil potential alterations of the protein tertiary structure in ice from binding of ANS (12)
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The sensitivity of the ANS binding method to the tertiary structure of proteins in ice should find practical utility in devising safer freeze-drying protocols of pharmaceutical proteins and testing the effectiveness of various stabilizing/ destabilizing agents employed in protein formulations
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Increased protein backbone conformational entropy upon hydrophobic ligand binding
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Ring**Institute of Food Research, Norwich Research Park, Norwich NR4 7UA, United Kingdom; and [dagger] Department of Food Processing, Tallinn Technical University, Tallinn 19086, EstoniaSubmitted April 8, 2005, and accepted for publication May 25, 2005
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The population of DNA molecules in coil or compact states was determined by an analysis of at least 100 DNA molecules
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The detergent-dispersed proteins also showed a K^sub d^(app) ~ 70 nM
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.Size exclusion chromatography was used to investigate the quaternary structure of GR (Fig
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.[FIGURE 4 OMITTED]Individuals became more thick bodied as they developed
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