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5 M, an increase in intensity of the fluorescence emission, along with a red shift from 327 to 336 nm was observed (Fig
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At the other end of the spectrum, recent experimental work and some modeling efforts suggest that a new paradigm is in order for denatured-state ensembles (100,101,130)
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Predicting the energetics of osmolyte-induced protein folding/unfolding
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Protein concentration was determined from the absorption at 280 nm and enzymatic activity was tested before and after neutron scattering experiments (36) at 25°C in a 25-mM phosphate buffer (pH 7
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The sliding process is likely to occur on a nanosecond timescale, although CaM:SEF2-lmp interactions may slow down this process in some instances
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In contrast, PLCβ^sub 2^ and its isolated PH domain, and the PLCβ chimera used here, binds to different membranes with little specificity (Wang et al
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