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Spectra were averaged over three scans for each sample containing 3 µM GR in 30 mM HEPES, at pH 7
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2 and 1 mg/ml were prepared and used for the Trp fluorescence analysis
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However, on increasing the denaturant concentration further, to 3
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Our presentation is organized as follows
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Experiments were carried out in the absence and in the presence of the effectors (at final concentrations of 2
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The transitions measured by CD became broader as the concentration increased, and although a small increase was observed when the concentration was raised (3 µM yielded T^sub m^ = 328
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In Monographs on the Physics and Chemistry of Materials
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5), 100 mM NaCl (referred to as annealing buffer) at a concentration of 1 µM donor strand, 5 µM acceptor strand, and 10 µM of additional strands (if applicable) and were annealed by heating to 70°C for 2 min and cooling to ambient temperature
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- Predicting the energetics of osmolyte-induced protein folding/unfolding
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. The V4 loop (residues 398-409), for which electron density is missing in all crystal structures, and the V3 loop, which has been proposed to undergo significant rearrangements upon CD4 binding, were modeled onto the gp120 core, together with the six core mutations of the LAI strain (98
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. For a given sequence, N residues long, we start with a random, sterically allowed conformation for the chain and calculate the inverse power potential energy U according to the formula shown in Eq
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