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. A further increase in quenching efficiency was observed at 7 M GdnHCl (squares, K^sub SV^ = 40 M^sup -1^), indicating that even at 3 M GdnHCl the tryptophan residues of GR were still significantly protected from iodide quenching relative to the fully unfolded protein
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. The native ACYI contained Class I (~60%) and II (~40%) fluorophores, but did not contain Classes A, S, or III (Fig
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. Probing the dimeric structure of porcine aminoacylase 1 by mass spectrometric and modeling procedures
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. 31nm and that of gSBA is ~4
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. Hence, one can evaluate the extent of the intersubunit interactions due to glycosylation
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. Glycosylation:heterogeneity and 3D structure of proteins
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. Temperature scans were performed using a constanttemperature cuvette holder connected to an external constant temperature circulator/bath (F25, Julabo, Seelbach, Germany; using a special Julabo bath fluid H10S)
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. The S^sup 2^^sub f^ and τ^sub if^ values extracted from M2II are presented in Fig
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. All measurements were performed at room temperature
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. Discrete coil-globule transition of single duplex DNAs induced by polyamines
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. This precisely exemplifies the general mechanism we have suggested, with the additional feature that one of the hypergutters has become so stabilized (and therefore so populated) that it attracts the label of "intermediate state"
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. Among the Piloto positive stations (n=20), P
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. Specific recognition of CD4 by gp120 is evidently the linchpin of the CD4-mediated viral infection pathway
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. One advantage of approaches based on the covariance matrix of atomic fluctuation is the possibility to compute this quantity for different subsets of atoms or degrees of freedom
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. Yet, the neglect of correlation between a particular subset of atoms and the rest of the molecule is a reasonable first-order approximation
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. The atoms of each residue (code: res); see Fig
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. We studied the effect of different solution thermal histories using Isopar G and cooling in an ice and water bath
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. Thus, within the framework of these simulations and the two-state model, we can estimate the forward rate constant (k^sub 1^) for the extended-undocked to quasi-docked reaction to be in the 10 × 10^sup 3^- 41 × 10^sup 3^ s^sup -1^ range and the reverse rate constant (k^sub -1^) in the 3
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. They were dehydrated sequentially in a series of graded alcohol washes, cleared in xylene prior to embedding in paraffin
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. 00PaperbackRM300Writing primarily for medical students, pharmaceutical reps, and practitioners in need of an update, McGavock (prescribing science, U
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. Principles of Fluorescence Spectroscopy
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. The far-UV CD spectrum of EI^sup sc^ at neutral pH showed the features of an α-helical protein with minima at 208 and 222 nm (41,42) (Fig
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. For water suppression, three successive sinc3 pulses of 19 ms were applied along with spoiling gradients of 6 ms and 120 mT/m
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. Target enzyme recognition by calmodulin: 2
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.The molecular details of how PLCβ interacts with Gβγ is unknown
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. In proteins with more complex suctures, other candidates suggest themselves, such as the orientation of helices with respect to β-sheets with which they are in contact in α/β proteins, and the relative orientations of β-turns and partially-folded β-sheets in all-β elements
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. After incubation at room temperature for 20 minutes, the eggs were washed with 200 [micro]L of PBS
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. 3 B) as described in the Materials and Methods section
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