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Furthermore it was demonstrated here in the case of homologous α-amylases, that accompanying studies with additional techniques (CD, static, and dynamic light scattering, see for example (14,36) for analyzing the compactness in terms of radii of gyration of unfolded states) can help to obtain a more meaningful interpretation of the tryptophan fluorescence data advance cash indiana payday .
To aid comparing the ANS binding propensity among the various proteins Table 2 lists the minimum number of binding sites (n) as well as the ratio n/Mw, normalization by the molecular weight taking into account that larger macromolecules, such as oligomers, have a greater probability to bind ANS simply for their larger mass advance cash indiana payday .
The Θ^sub 222^(t) data show slow unfolding on a timescale of 10^sup 2^-10^sup 3^ s (as compared to 10^sup -8^-10^sup -10^ s for the helix-to-coil transition in solution; Yang and Gruebele, 2003), implicating high free-energy barrier ΔG* advance cash indiana payday .
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