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The biological relevance of the partially folded species at acidic pH is unknown, and we do not know whether the residual secondary structure detected by CD and FTIR (Table 2) was native-like
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In short, apoptotic cells must express clear-cut "eat-me" signals (Fig 2, top, A)
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Partition coefficient calculation The partition coefficients were calculated with the ClogP program (available at http://www
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HPRI-CT-2001-50035 and RII3-CT-2003-505925
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Partially folded intermediates in insulin fibrillation
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5] t, t [greater than or equal to] November 1995 (3)Under the null hypothesis that Type 1 RP did not affect quantity, [[gamma]
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. This investigation aims to complement that initial study by addressing the following issues: i), establish whether the perturbation of protein structure reported for azurin is general to other proteins; ii), inquire on potential correlations between the susceptibility to the ice-induced perturbation and specific features of the macromolecule such as thermodynamic stability, internal flexibility, internal cavities/hydration, and covalent links between strands; and finally iii), test for a specific vulnerability of multimeric proteins to freeze damage that may be linked to the disruption of quaternary structure
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