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. Compared to native GR (circles), addition of 3 M GdnHCl (triangles) led to a significant increase in quenching efficiency, as indicated by an increase in K^sub SV^ from 12
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. The average shape of a denatured protein is that of a prolate ellipsoid We computed the ensemble-averaged asphericity values for each of the 23 sequences in the EV limit and the resultant data are shown as cross marks in Fig
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. Azurin WT and mutants I7S, F110S, and C3A/C26A were prepared following published protocols
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. Principles of Fluorescence Spectroscopy
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. coelicolor, EI^sup sc^, indicate that its thermal denaturation behavior is different from that of other EI proteins, since only one transition is observed (23)
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