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No changes in anisotropy were observed up to 2 M GdnHCl
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Kratky profiles for proteins in the EV limit were compared to those of folded proteins and ideal, freely rotating chains (43)
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2-cm cell at a protein concentration of 0
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As a consequence the corresponding emission spectra that were measured during unfolding appear completely different (Fig
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We conclude that stabilization, and corresponding effects on the MSDs of HuBChE, by solvent/buffer and by the covalently bound inhibitor involve two different mechanisms
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Prear, The Mechanics of Solder Alloy Wetting and Spreading (New York: Van Nostrand Reinhold, 1993), pp
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Other diseases are caused by a gain-of-toxic-function mechanism often including aggregation of misfolded proteins
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By comparing the volume change of PLCβ-Gβγ alone and in the presence of Gα^sub s^(GDP), we can estimate the volume of activation of PLCβ^sub 2^ by Gβγ-subunits
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Large unilamellar vesicles (LUVs) were freshly prepared by extrusion through a 100-nm pore filter before every experiment
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Similar unfolding below 25°C was detected in the earlier studies of human lipid-free apoA-2 at much higher protein concentration (>0
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Further cooling leads to a steep reduction in the light scattering (and thus in the particle size) between 34 and 24°C
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Sagittal otoliths were removed and stored dry for age determination
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Manuscript approved for publication 2 March 2004 by the Scientific Editor
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Ribozyme constructs missing the substrate strand were prepared in annealing buffer (missing NaCl and supplemented with 12 mM MgCl^sub 2^) at a concentration of 2 µM of each strand and annealed by heating to 70°C for 2 min and cooling to ambient temperature
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About one third of the sample were male (n = 56)
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