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The fully deuterated samples were obtained by incubating the protein solutions in a water bath at 50°C for 15 min, and then the samples were cooled to room temperature and lyophilized
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Interestingly, the difference in T^sub m^ of the two forms is ~26°C, and this estimates the extent of stability imparted by the oligosaccharide chains to the stability of gSBA
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These changes were probably due to a histidine residue, which titrates in that pH region (43)
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This work was supported by an Atorvastatin Research Award (R
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The concentration of Na ion fluctuates during the cell cycle, with peaks in the mitosis and synthesis phases, whereas the concentration of K+ does not change (Warley et al
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Importantly, all probes show different kinetics, with varying amplitudes and rates during the reaction (Table 1)
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