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We examined two single point cavity-forming mutants, I7S and F110S, whose internal cavities of 40 and 100 [Angstrom], respectively, appear to be fully hydrated (18), and the double mutant C3A/C26A in which the disulfide bridge linking strands β1 and β2 has been severed
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Here, we compare the ANS binding capacity of the four azurin species to inquire how these modified features modulate the ice-induced perturbation of tertiary structure
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On a molar basis, the greatest apparent affinity and the largest values of H are exhibited by the tetramers LDH, YADH, and ALD, the smallest by the monomers azurin, β-gal, and α-amylase
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These calculations indicate that the membrane is ~2 orders of magnitude more permeable to diminazene than to aceturate
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At pHs in the vicinity of the isoelectric point (pH 5
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The quenched Trp fluorescence in the presence of ANS and Bis-ANS (Fig
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Careful double mutant studies would be required to identify such nonnative contributions unequivocally
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