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with a final concentration of 1
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The curves from the diluted solution (data not shown) were similar to those presented in Figs
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The other major change in a fluorescence signal upon conformational changes of the protein is related to solvent relaxation, which determines the magnitude of the stokes shift
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For diffusion spFRET measurements, the resulting ribozyme complexes were diluted with annealing buffer (missing NaCl and supplemented with 0
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