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. interactions between EIN and EIC in the whole intact EI have been suggested (44)
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. In the last decade, strong binding of ANS to molten globule states of proteins has been linked to the loss of tertiary structure (7), and since then the method has been applied to characterize transient states in protein denaturation (8-11)
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. Our gradient coil capacity allows still higher gradient strengths, but these cause larger eddy currents and possibly also signal distortions in MRS experiments
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. For example, the calculated ratio of k^sub cat^ (DmAChE/HuBChE) for hydrolysis of various substrates at 25°C is 1
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. Pressure- and heat-induced inactivation of butyrylcholinesterase: evidence for multiple intermediates and the remnant inactivation process
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. Slowing down the scan rate also leads to a reduction in the CD amplitude in the cooling curves below 80°C (Fig
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. As an example of the type of fine-structure predicted, the model contains a natural explanation of the curvature seen in plots of the denaturant dependence of folding rates
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.A control comparison of HuBChE-H2O and HuBChE-D^sub 2^O (not shown) revealed identical MSDs below 260 K, but higher MSDs of the H2O-hydrated sample at higher temperatures
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