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. This is the case if at least one major component of the surrounding solvent is chemically equivalent to the main repeating unit of the polymer making chain-solvent contacts preferable to chain-chain contacts (4,37)
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. To test whether there is a relationship between the extent of ANS binding induced by freezing and the instability of azurin to the freeze-thawing process, we have determined the degree of irreversible denaturation of WT and mutant species when subjected to repeated freeze-thawing cycles
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. Are these activity differences on the millisecond timescale reflected in differences in atomic thermal fluctuations on the pico- to nanosecond timescale?3
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. From the distribution of (τ^sup o^^sub m^) values, the shape of the diffusion tensor can be determined (30), whereas the degree of anisotropy can be gauged from the maximum and minimum (τ^sup o^^sub m^)^sub app^ values
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.Manuscript submitted 4 April 2003 to Scientific Editor's Office
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