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No model based on protein conformation distribution was used to analyze or interpret these results in more detail; however, such decay times and relative amplitude to the overall fluorescence decay are useful parameters when comparing samples of different chemical history or taken as well as in interpreting relative contributions to steady-state fluorescence data america bank boat loan .
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Once protein unfolding reached completion in the first jump, the sample was cooled and incubated at 25°C for 1000 s, which was sufficient for completion of the helical refolding (as indicated by flattening of the Θ^sub 222^(t)) but not for the disk reconstitution (as indicated by DSC, EM, and gel electrophoresis) america bank boat loan .
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