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. Second, unlike hard-sphere potentials, which stipulate that all sterically allowed conformations are isoenergetic, soft-core potentials encode the requisite conformational specificity (48,53,58)
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. This helix-loop-helix motif folded against the ban-el, covering a hydrophobic patch (which includes Tip^sup 461^): the motif was linked to the barrel by two salt bridges, namely, Arg^sup 537^-Asp^sup 467^ and Arg^sup 546^-Asp^sup 459^ (Fig
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. The aromatic chromophore 1-anilino-8-naphthalene sulfonate (ANS) is feebly fluorescent in water, but its spectrum is blue shifted and its intensity is dramatically increased in nonpolar solvents or when it binds to nonpolar sites of proteins (4,5; for a general review, see Slavik (6))
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