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Despite the large amounts of ACYI that exist in mammalian kidney and liver, the reactivation of ACYI is particularly difficult in vitro (19-21), which suggests that the folding of ACYI in vivo might be assisted by the possible intracellular molecular chaperones and/or osmolytes
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The initial decrease in fluorescence intensity takes place despite the solidification of water raised by ~55-fold the concentration of ANS in the liquid water pool of ice (12)
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The rate-determining "gutter" dimensions lie orthogonal to other "zipper" dimensions describing the local formation of secondary structure, that are characterized by a continuous folding funnel
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