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Is protein folding hierarchic? II angeles car loan los .
(5) Clare DA and Swaisgood HE, 2000 angeles car loan los .
Measuring the confomiational stability of a protein angeles car loan los .
For this purpose the same experimental setup (temperature controlling unit, quartz-cuvettes with 1 cm optical pathlength) was used as for fluorescence studies angeles car loan los .
Although suitable data are not available to establish if there is a direct correlation between the above ANS binding capacity and the actual instability to freeze-thawing (depending on the protein, irreversible damage has been estimated by diverse criteria such as loss of enzymatic activity, degree of aggregation, and loss of secondary or tertiary structure and, further, the outcome is generally influenced by the rates of freezing and thawing), it is a common observation that multimeric enzymes tend to be less stable to freezing than their monomeric counterpart (17) angeles car loan los .
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The spatial resolution of MRI typically ranges between millimeters and hundreds of microns (4,5), whereas MRS-which generates signal from nonwater protons whose concentrations fall far below those of the water protons used in MRI-requires voxel sizes of tens of millimeters for humans to a few millimeters for smaller animals like rodents (6,7) angeles car loan los .
As the SNR of NMR experiments increases with magnetic field strength, localized spectroscopy experiments at higher strengths could be promising because they could reduce the minimum detectable drug concentration in the cell angeles car loan los .
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Non-denaturing gel electrophoresis of lipid-free and DMPC-associated proteins was carried out by using 8-25% polyacrylamide gradient gel (PHAST system, Pharmacia, Peapack, NJ) with Coomassie staining angeles car loan los .
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