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. Since K^sub SV^ equals the product of the bimolecular quenching rate constant (k^sub q^) and the excited-state lifetime of the fluorophore ([tau]), the value of K^sub SV^ obtained from steady-state fluorescence measurements is dependent on the average lifetime
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. The thermal dependence of ACYI activity was measured by incubating the enzyme solutions at given temperatures for 30 min, and then the activity assay was performed by mixing the enzyme solutions and reaction buffers preheated at the same temperatures
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. Furthermore, of these 188 proteins, 104 contain both forms of glycosylation (6)
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. None of these models can provide an accurate description of conformational ensembles accessible to highly denatured proteins since they explicitly disregard the effects of correlated fluctuations imposed by two-body EV interactions
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. Cheong, unpublished), applications of localized MRS to the above-mentioned processes would make embryonic development accessible to noninvasive in vivo investigations
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. The exchange data for all residues are summarized in Fig
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. AFM operation and image analysisThroughout the measurements, the tapping-in-air AFM mode was used
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. ofYear Cruise DatesstationsIchthyoplankton surveys1978-79 SM-IX 26 Dec-07 Jan 281982EH-05/8219 Nov-03 Dec 651983EH-01/8314 Jan-26 Jan 431985OB-02/8525 Mar-04 Apr 301986OB-01/8620 Jan-03 Feb 40OB-07/8609 Dec-22 Dec 431991OB-07/9101 Nov-11 Nov 351995OB-14/9505 Dec-18 Dec 751996EH-17/9612 Dec-21 Dec 181998OB-10/9807 Dec-20 Dec 871999OB-09/9911 Dec-17 Dec 152000OB-14/0009 Dec-21 Dec 272001EH-01/0106 Jan-29 Jan 28OB-02/0112 Feb-25 Feb 40OB-13/0110 Nov 13 Nov 18Posttransition juvenile trawls1992EH-02/9202 Mar-21 Mar 451998EH-04/9801 Apr-10 Apr 411999EH-04/9920 May-31 May 562000OB-05/0001 Jun-20 Jun 1122001OB-02/0112 Feb-25 Feb 23Lat
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. The polymer solution was poured into a dynamic light scattering (DLS) cell and cooled for 1 hour at 25°C
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. Since the docked and undocked peaks are well resolved, any interconversion between the two conformers must occur more slowly than the ~280-µs average burst duration of the single-molecule measurements (see below)
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. The results? An increase in both upper and lower body (whichever area you exercise) muscle size and circumference
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. The IR difference absorption spectra were calculated by subtracting the spectra at given temperatures from the spectrum recorded at 30°C (Fig
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. To better visualize the sequential events occurring during ACYI thermal unfolding, 2D IR synchronous and asynchronous plots were constructed
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. Only the 1g9f entry shows up the two proximal GIcNAc residues
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.Φ-Values: normative contributionsAs a second general prediction, and as an example of the specific calculations possible with the model, we examine the important question of Φ-value analysis and its interpretation
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