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. 14), also known as Acylase I (ACYI), is a homodimeric enzyme containing one zinc ion per subunit (4-6)
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. For the longest sequence in our data set-the sequence of villin-for which N = 126, generation of the desired conformational ensemble takes ~ 20 h on a single 2
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. The backbone structures of the CaM N- and C-terminal domains within the CaM:SEF2-1mp complex are expected to be very similar to those in other CaM structures
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. In more biochemical language these structures might be exemplified by cooperative secondary structure formation alongside native or near-native distant contacts in α-helix bundle proteins (Myers and Oas, 2001), or simultaneous folding and anion binding (Henkels et al
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. Docked fractions calculated from relative areas of the peaks are plotted in Fig
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