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. At temperatures above 66°C, the content of the Class I fluorophore decreased continuously, whereas the Class III component increased
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. The protein concentration used for the studies is 0
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. The free energy of unfolding calculated for rSBA and gSBA as per LEM model are 41
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. as in a helix-coil transition, then no nonnative local contacts would have to he broken upon folding
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. Protein concentration was calculated by using the extinction coefficients of Trp, Tyr, Cys, and Phe residues (24)
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. No significant difference in the circular dichroism spectrum was seen in either the far UV (190-230 nm) or the near UV (260-300 nm) before and after the neutron scattering measurement
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. In soman-aged HuBChE, the organophosphoryl moiety remains covalently attached to the unfolded protein, and the salt-bridge between the protonated imidazole ring of His-438 and the negatively charged phosphoryl oxygens may contribute to the observed shift in the onset of unfolding to a higher temperature compared to the native enzyme
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. Post-translational modifications of Drosophila acetylcholinesterase
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. Deriving the activation barriers assuming a two-state exchange is likely to be a significant simplification, as the conformational landscape may be much more complex
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. , when the CaM:SEF2-1mp interaction brakes, one side of the CaM dimer interaction also momentarily brakes
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. ApoA-2 has particularly large apolar helical faces (50% of the surface as compared to ~30% in apoA-1 and apoC-1) which, along with higher hydrophobicity of these faces, is thought to account for stronger association of apoA-2 with HDL (Brasseur et al
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. Formation and metabolism of pre-β-migrating, lipid-poor apolipoprotein A-I
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. The rate determining step is not the rapid formation of local secondary structure, nor the diffusion of subdomains per se, but the organization of large pieces of secondary structure into the same topological configuration as the native state, which thereafter is able to form rapidly
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. brasilianus posttransition juveniles differ in their head shape, pigmentation pattern, and in the number of spines of the dorsal fin
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. 12 kJ K^sup -1^ mol^sup -1^; see Fig
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. Error analysis of configurational entropiesThe configurational entropy differences between complex and free forms reported above were obtained from the entropy differences at 8 ns (the full trajectory length used for analysis)
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. Four isotactic polypropylene (IPP) polymers (Fina Oil & Chemical Company, Laporte, TX) and four poly(ethylene-co-vinyl acetate) (EVA) polymers (Millenium Petrochemical Corporation) were used as received as binders for the toners (see Table I)
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. Age-related differences in the timing of spawning have been observed in many fishes; usually larger, older fish spawn earlier (Simpson, 1959; Bagenal, 1971; Berkeley and Houde, 1978; Shepherd and Grimes, 1984; Lambert, 1987), but in some cases younger fish spawn earlier in the season (Hutchings and Myers, 1993)
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. , unequal elevations) which necessitated that the data be fitted with a parallel-lines multiple linear regression model (Fig
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. 1991, Parmalee 1954, Zimmerman et al
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. has been heated, evidence on its outcomes is limited (Kanavos and Reinhardt 2003)
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. Exemption for a "second line restricted" NSAID (nabumetone, piroxicam, tenoxicam, tiaprofenic acid, tolmetin, sulindac, ketorolac, or diclofenac potassium) required failure on a first line restricted NSAID
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. The sample with the protein and the effectors was incubated for a minimum of 2 h at room temperature
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. two peaks with different thermodynamical properties are observed, one corresponding to EIN (with pH-independent T^sub m^, values) and a second corresponding to EIC (with pH-dependent T^sub m^-values) (6)
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. The obtained peak maxima around 336-338 nm indicate rather small red shifts (~2-3 nm) with respect to the native state (see also Fitter and Haber-Pohlmeier (14))
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. We first analyze the latter class and demonstrate that additional binding of ANS in ice is due to the creation of new binding sites rather than to a more complete saturation of preexisting weak sites after the large freeze-induced concentration of the ligand
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.5 × 10^sup -11^ m^sup 2^s^sup -1^, in agreement with previous measurements carried out under similar conditions and concentration (33)
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. The final solution contained 100 g/L PEG, 2 × 10^sup -7^ M DNA (in basepairs), 2 × 10^sup -7^ M DAPI and an appropriate amount of alkali chloride
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. Zinchenko and Kenichi YoshikawaDepartment of Physics, Graduate School of Science, Kyoto University, Sakyo, Kyoto, JapanSubmitted December 1, 2004, and accepted for publication March 8, 2005
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. It should however be noted that Bis-ANS still fluoresces very strongly in the presence of large aggregates (Figs
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. This permitted sufficiently accurate height determination (maximal vertical resolution was 0
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