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. The preceding study (12) has demonstrated that the creation of hydrophobic ANS binding sites in ice, accounting for the spectral blue shift and intensity enhancement, is owed to partial disruption of the protein tertiary structure triggered by the solidification of water
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. The spectrum of lipid-free apoA-2 at 25°C is invariant in the protein concentration range from 5 to 20 µg/ml and suggests 30% α-helix (Fig
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. Differential light scattering and absorption flattening optical effects are minimal in the circular dichroism spectra of small unilameHar vesicles
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. This suggests that the aggregation process is essentially completed after 1 h at 70°C
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. semifasciata at early stages indicate the existence of at least three offshore reproductive grounds between 42-43[degrees]S, 43-44[degrees]S, and 44-45[degrees]S, and a delayed spawning pulse in the southern stocks
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