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The modeled structure of the EIC^sup sc^ monomer differed from that of EIC^sup tt^ (11) only in a deletion in the loop between α-helix 4 and β-strand 5, which is part of neither the active center nor the dimerization surface apply for school loan .
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They are clustered in the CaM:SEF2-1mp binding interface, the CaM:CaM dimer interface, and in the flexible helix connecting the CaM N- and C-terminal domains, and have similar exchange times (~50 µs), suggesting a cooperative mechanism probably caused by protein:protein interactions apply for school loan .
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Large unilamellar vesicles (LUVs) were freshly prepared by extrusion through a 100-nm pore filter before every experiment apply for school loan .
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