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. Incoherent inelastic neutron scattering experiments probing the vibrational density of states are scarce but seem to favor an increase of low-frequency, collective motions resulting in increased protein flexibility upon ligand binding (10)
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. Details about site-directed mutagenesis, protein expression, isolation, and purification of mutants I7S, F110S, and C3A/ C26A have been described elsewhere (22,24)
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