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Inactivation-reactivation of two-electron reduced Escherichia coli glutathione reductase involving a dimer-monomer equilibrium
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5 as shown by scanning microcalorimetry, viscosimetry, nuclear magnetic resonance, and circular dichroism spectrometry, electrometric, and calorimetric titration (Griko et al
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Although gSBA was seen to demonstrate very high conformational stability, rSBA exhibits comparatively much lower firmness to resist the structural perturbation induced by the denaturant guanidinium hydrochloride
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Similarly, the free energies of unfolding of C^sub H^2 antibody domain and pliage P22 coal proteins are, respectively, 3
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We have compared our results to those from previous work (48,58) where no cutoffs were used
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(7)While it takes time, products featuring many of these beneficial ingredients are finding their way to market
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To quantify the secondary structure components, the amide I' band was decomposed into its constituents by curve-titling (based on a combination of Gaussian and Lorentzian functions)
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^sup 15^N chemical shifts were indirectly referenced using the gyromagnetic ratio of ^sup 15^N/^sup 1^H (18)
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DISCUSSIONThe influence of the chemical nature of a monocation on its DNA compaction ability is clearly demonstrated by the observation of the collapse of single DNA molecules by fluorescent microscopy
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This dependence of the binding of monocations is usually explained from the viewpoint of a decreasing hydrated radius of monocations in aqueous solutions (Ivanov et al
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We also employed the classic and widely used, although poorly understood, amyloid fluorescence dye ThT, which is known to interact with amyloid fibrils (Naiki et al
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Sequence-dependent denaturation energetics: a major determinant in amyloid disease diversity, fror
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The prolonged 10-min incubation at 60°C promotes further elongation of the fibers even up to 2
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This, in fact, is direct evidence supporting the model of amyloids as waterfilled nanotubes (Perutz et al
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semifasciata in northern Patagonia (42-44[degrees]S) peaks in November and December (Elias and Burgos, 1988; Macchi et al
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However, the oxidation of milkfat can occur even at temperatures as low as that of stored ice cream
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For histogram simulations of the SL 4WJ at 10 mM Mg^sup 2+^, the state of the ribozyme system was allowed to stochastically switch between the extended undocked and quasi-docked states according to the assumed forward and reverse rate constants (see Fig
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The samples were subsequently fixed using 1% osmium tetroxide in 0
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At each timepoint three replicate samples were analysed providing a total of nine replicate values from three separate experiments
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Louis, Illinois, is a shuttle ride away from the Casino Queen riverboat and the closest campground to St
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Their ages ranged from 18 to 25, with a mean age of 20
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. The 2D IR plots were presented as contour maps produced by drawing the contour lines every 10% off from the maximum intensity of the whole correlation map
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. In EI^sup ec^, the thermal midpoint of the transition monitored by fluorescence is concentration-dependent (52), but the slopes of the native baseline are smaller due to the fact that two tryptophan residues are observed in the C-terminus of EI^sup ec^
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. The results clearly indicate that the extent of azurin denaturation-aggregation is correlated to the value of n increasing from WT to mutant proteins and that among the latter species it is significantly larger with the cavity-forming mutants I7S and F110S relative to compact C3A/C26A
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.Transmission electron microscopyFive microliter aliquots from a TTR misfolding reaction were withdrawn at different time points and were placed on a carbon coated copper grid for 2 min
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. Pressure has profound effects on the highly compressible membrane interior promoting phase transition to gel phases and, for the studies presented here, the phase transition will occur ~1
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. Far-U V CD spectra (185-250 nm) were recorded from solutions of 20 µg/ml protein concentration placed in 5-mm-pathlength cells, which are standard condition in our CD experiments
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. Specifically, focusing on the prefibrillar amorphous aggregates occurring 30 s after elevating temperature to the nucleation-enhancing 60°C, revealed the tendency of the globule-shaped oligomers to queue and assembly into elongated forms
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.Protein Folding in High-Dimensional Spaces: Hypergutters and the Role of Nonnative InteractionsMcLeish, T C BABSTRACTWe explore the consequences of very high dimensionality in the dynamical landscape of protein folding
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