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1 M Tris-Cl, at pH 8
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, Mayor Michael Albano to continue importing drugs from Canada for city employees and retirees
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. Our results show that dimeric EI^sup sc^ acquired a folded conformation at physiological pH: however, 8-anilino-1-naphthalene-sulfonic acid (ANS)-binding experiments, FTIR, and CD measurements suggested that the protein populated a molten-globule-like species at low pH
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. ANS binding experiments, extended to a set of proteins including serum albumin, α-amylase, β-galactosidase, alcohol dehydrogenase from horse liver, alcohol dehydrogenase from yeast, lactic dehydrogenase, and aldolase, confirmed that a stressed condition of the native fold in the frozen state appears to be general to most proteins and pointed out that oligomers tend to be more labile than monomers presumably because the globular fold can be further destabilized by subunit dissociation
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