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. Attributes of Glycosylation in the Establishment of the Unfolding Pathway of Soybean AgglutininSinha, SharmisthaABSTRACT Soybean agglutinin (gSBA) is a tetrameric legume lectin, each of whose subunits are glycosylated
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. A figure presenting the matrix-assisted laser desorption ionization mass spectra of the glycosylated and nonglycosylated form is shown in the Supplementary Material
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. To aid comparing the ANS binding propensity among the various proteins Table 2 lists the minimum number of binding sites (n) as well as the ratio n/Mw, normalization by the molecular weight taking into account that larger macromolecules, such as oligomers, have a greater probability to bind ANS simply for their larger mass
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. EXPERIMENTAL PROCEDUREMeasurement of Contact Angle and Wetting ForceThe wettability test was performed using the solderability tester MENISCO ST 50 with computer control and data acquisition functions
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. Equation 2 implies that when contact angle θ^sub c^ increases, the surface tension force moves toward negatives due to the cosine value of θ^sub c^, resulting in the negative wetting force
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. Given δ^sub 1,2^ and a reasonable maximum of Δ^sub ex^ (~6 ppm), a correlation plot of R^sub ex^ vs
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. This is of course a restatement of Levinthal's paradox (Levinthal, 1969; Karplus, 1997), but a helpful one, in that the two necessary assumptions for the paradox to arise are clearly seen
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. The carrier is used to impart a triboelectric charge to the toner when toner and carrier are mixed to produce the developer
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. But, after incubation at 20[degrees]C for 24 hours until the late stage, all six eggs identified as P
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. RNA conformation and folding studied with fluorescence resonance energy transfer
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. Address reprint requests to Makoto Suzuki, Department of Metallurgy, Graduate School of Engineering, Tohoku University, Aoba-yama 02, Sendai, 980-8579, Japan
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. N-Acyl-L-aromatic amino acid deacylase in animal tissues
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. Of particular interest is the value of R^sub o^, which is related to the persistence length and also provides a measure of the goodness of the solvent because R^sup 3^^sub o^ quantifies the average volume per residue set aside by the chain for interactions with the surrounding solvent (38,41)
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. Three pHs were chosen based on the steady-state fluorescence results: pH 5, close to the midpoint of the titration observed in ANS-binding and fluorescence intrinsic experiments; neutral pH, where the intensity of steady-state fluorescence experiments was at its maximum; and pH 12, in the basic region
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. Backbone dynamics of free barnase and its complex with barstar determined by 15N NMR relaxation study
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. We remark that in both these cases, the stabilized hypergutter provides an arena in which "diffusion-collision" calculations can operate within a molten globule, so constituting a significant generalization of that model to nonspatial degrees of freedom (Zhou and Karplus, 1999)
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. In consequence there are contributions to measured Φ-values that arise from this "preexponential" effect
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. The enthalpic change upon gp120/CD4 complex formation, calculated as the difference in proteinprotein plus protein-solvent potential energy between the simulations of the complex and of the separate proteins (-127 kJ mol^sup -1^ at 300 K with a standard error, SE, of 11 kJ mol^sup -1^), is of the same order of magnitude as the experimentally determined value (-259 ± 13 kJ mol^sup -1^ at 310 K; Myszka et al
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. Specificity of the antibodies was examined by using the eggs shown in Table 2
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.t] = 1] - E[[DaysNSDPop
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