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These two bands have been proposed to be the sign of the formation of intermoltcular β-sheet in aggregates (42, 43)
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The PTS involves a cascade of phosphoryl-transfer steps from phosphoenolpyruvate (PEP) via phosphointermediates of the general cytosolic non-sugar-specific phosphotransferases enzyme I (EI) and the histidine-phosphocarrier (HPr) to sugar-specific enzyme II permeases
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5 mL samples were placed in cylindrical spectrosil cuvettes, 4 mm inner diameter, and rapidly frozen in liquid nitrogen
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In either case the conclusion is that in the frozen state the polypeptide structure of the putative binding sites has changed, becoming unable to host ANS or to enhance its fluorescence yield, as when the probe surrounding changes from rigid to mobile (26)
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laevis oocyte arrested in prophase I
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In considering data obtained by x-ray crystallography, Frauenfelder and Petsko (11) found that binding of oxygen was accompanied by a reduction in B-factors of residues close to the heme pocket in myoglobin
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Residues 14, 19, 51, 55, 76, 92, and 118 show a relatively strong decrease in exchange rate upon change in temperature (f = 0
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Samples containing 1-anilinonaphthalene-8-sulfonate (ANS) and Bis-ANS were excited at 290 nm, 370 nm, and 403 nm
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This approach has been employed here to deconvolute the fibrillar structure shown in Fig
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Negative activation enthalpies in the kinetics of protein folding
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major (20 April, 1995) could react with the antibodies obtained as clearly as the recently collected eggs of P
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After initial fixation the tissue samples were stored in a 0
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17) were significantly more competitive than women (M = 55
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The younger generation tends to be optimistic about this plan
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However, evidence inconsistent with such weighted utility models has also been reported
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. In these studies, we observe that mostly the mannose residues are involved in the intersubunit interactions
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. The PTS involves a cascade of phosphoryl-transfer steps from phosphoenolpyruvate (PEP) via phosphointermediates of the general cytosolic non-sugar-specific phosphotransferases enzyme I (EI) and the histidine-phosphocarrier (HPr) to sugar-specific enzyme II permeases
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. The influence of solvent composition on global dynamics of human butyrylcholinesterase powders: a neutron scattering study
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. The parameters that can affect the wettability were investigated through modeling and are discussed elaborately
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. This is called "wraparound" binding because the two domains in CaM wrap around the α-helical target (8,9)
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. NMR study of Brownian tumbling and internal motions in proteins
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.02-30 Hz, a viscous liquid-like response
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.5-30 min): formation of soluble oligomers; 3), second aggregation phase (30 min-20 h): generation of larger aggregates including spherical aggregates and short protofibrils; and 4), maturation phase (>20 h): generation of more extended fibrils
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. The majority of HDL contains both apoA-1 and apoA-2 (LpA-1/A-2); most of the remaining particles contain apoA-1 but not apoA-2 (LpA-1), and a small fraction contains only apoA-2 (LpA-2) (Cheung and Albers, 1984; Pastier et al
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. Appending the free and complex trajectories of CD4 gives rise to a small stepwise increase of the entropy buildup of the backbone atoms (Fig
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.Since the docked and undocked peaks are well resolved, any interconversion between the two conformers must occur more slowly than the ~280-µs average burst duration of the single-molecule measurements (see below)
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. All SL constructs show just a single peak in the FRET efficiency histograms, with no distinct docked peak, as expected (Fig
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. By contrast, abundant matrix and collagen fibrils were observed in the pericellular region of pellets cultured in DMEM/ITS+/TGF-[beta]1 (Figs 5b and c)
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. Journal of Social Psychology, 133, 507-511
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