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Is protein folding hierarchic? II
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Reference deleted in proof
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(19) In an elegant series of studies, Brown and colleagues (19) demonstrated that homophilic ligation of CD31 on viable leukocytes causes them to detach from macrophages
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The availability of intracellular spectra opens a new window for the investigation of intracellular processes, since the spatial and temporal fingerprints of nonwater molecules, be they physiological cellular components or external substances, can directly be followed on a subcellular scale
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MATERIALS AND METHODSEnzyme purification and characterizationHuBChE was purified to homogeneity from outdated human plasma according to an improved protocol based on that of Grunwald et al
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Hydrodynamic calculations suggest that this can be attributed to motions of the N- and C-terminal domains of the CaM dimer in the complex
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To enable conformational characterization of protein isolated from biological specimens such as samples from patients, noncovalent recognition using extrinsic dyes is necessary
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The pleckstrin homology domains of phospholipases C-β and -Δ confer activation through a common site
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The importance of diffusive searches is unsurprising, since under biological conditions, all candidates for energetic interactions, including electrostatics, are locally screened to a few angstroms: much smaller than the dimensions over which sections of protein must move to find their native configurations
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3 kJ K^sup -1^ mol^sup -1^
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