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The fully deuterated samples were obtained by incubating the protein solutions in a water bath at 50°C for 15 min, and then the samples were cooled to room temperature and lyophilized
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A comparison of the δ and S values of proteins in the EV limit to those of folded proteins is shown in Fig
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The results are shown in Fig
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Tran and Rohit V
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In the modeled structure, there was a long α-helix that is supposed to serve as a linker to the EIC domain
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A sample of ~200 mg DmAChE was dialyzed against, and then lyophilized from 0
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The cross sectioned samples were then finally gold coated and prepared for analysis
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The error in the R^sub 1^ and R^sub 2^ values was defined as the standard deviation between three curve fittings, one with all time points and two fittings with reduced data sels containing every second time point, which were shifted either zero or one time point in the relaxation series
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Moreover, protein unfolding and dissociation in this transition lead to thermodynamically irreversible disk fusion, as indicated by the DSC, EM, and gel electrophoresis data (Fig
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Although less confined than CD4-F43, it is in close contact (In addition to the residues that are directly involved in intermolecular contacts or are in close proximity to the intermolecular interface, there are a few residues in gp120 that show large intraresidue entropy changes upon complexation (see Fig
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Although their longevity and low rate of natural mortality is presumed to be an adaptation to allow successful reproduction over their lifespan despite long periods between favorable environmental conditions, it also makes them more susceptible to overexploitation
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Preparation of a highly specific monoclonal antibody to HLA antigen
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9 B, we plot a simulated histogram (bold line) using an equilibrium constant of 3 (in favor of the quasi-docked conformation), and forward and reverse rate constants of 20 × 10^sup 3^ and 6
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Journal of Personality and Social Psychology, 33, 435-441
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