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These observations suggest that FAD binding is unlikely to play a critical role in the process of GR folding/assembly
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Kinetics and energetics of subunit dissociation/unfolding of TIM: the importance of oligomerization for conformational persistence and chemical stability of proteins
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Comparison of the dashed curve to the other distributions reveals two features of the end-to-end distance distribution in the EV limit
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Finally, we studied the conformational and stability properties of EI^sup sc^ in the presence of the effectors (i
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The pronounced blue shift for native proteins in NDSB solutions is most probably related to the fact that NDSB interacts with the surface of the proteins which causes a more hydrophobic nonpolar environment of surface exposed tryptophan residues
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Two ml of a buffered BLG solution was dialyzed against buffered PEG solutions of known osmotic pressure (15,16) at 20°C for 24 h
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These results suggest that activation of PLCβ by Gβγ is conferred by a small (i
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The pressure cell was inserted into the optical module of the fluorometer allowing for fluorescence measurements under pressure
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The Gβγ-subunits used in this study are geranylgeranylated, whereas Gα^sub s^-subunits are dually palmitoyated (Kleuss and Krause, 2003: Wedegaertner et al
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The first peak is centered at 28°C and reflects DMPC phase transition in intact disks that is not detected by CD (Fig
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The RNA strands were deprolected, purified by denaturing urea gel elcctrophoresis, desalted and subsequently purified by reversed-phase high-performance liquid chromatography utilizing an eluent system of acetonitrile and aqueous triethylammonium acetate (100 mM, pH 7)
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Paul Grootendorst is supported by a career award from the Rx&D Health Research Foundation and the Canadian Institutes of Health Research (CIHR)
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