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5 M guanidinium hydrochloride (in 10 mM Tris buffer (pH 7
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Processes that disrupt or uncouple this balance have the potential to alter normal cell turnover, ultimately resulting in the induction of lung pathology and disease
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Retrospective birth dating of cells in humans
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These findings are supported by MD simulations that suggest that the flexibility of protein residues may either decrease or increase upon ligand-binding (9)
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9 µeV, corresponding to a time window of ~730 ps; accessible Q-range: 0
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Furthermore, several residues in CaM also undergo conformational exchange on a timescale of ~50 µs
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0 do not support this explanation (Fig
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5) or 40 mM Na-acetate (pH 3
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The integrated spectra were corrected by the fluorescence of each probe in the absence of protein and normalized to the recorded maximum intensity of each probe during the misfolding reaction
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Fluorescence anisotropy data from Bis-ANS bound to the different unfolded and aggregated states showed modest anisotropy values (compared to when bound to the thyroxine binding site in the native TTR tetramer), revealing that Bis-ANS docs not penetrate the protein but is more loosely associated to the protein surface (data not shown)
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It should however be noted that Bis-ANS still fluoresces very strongly in the presence of large aggregates (Figs
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MATERIALS AND METHODSPeptides and proteinsThe synthetic peplide corresponding to residues 86-105 of Gβ^sub 1^ (NH^sub 2^-TTNKVHAIPLRSSWVMTCAY-CONH^sub 2^) and the corresponding control peptide that does not activate PLCβ (Gβ86-105(M101N)) were gifts from Dr
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The reported partition coefficient refers to the lipid concentration at which the amount of bound versus unbound protein (K^sub p^ = [P^sub b^]/[P^sub un^]) is identical
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Even though the abundance and distribution data used in our study came from cruises that targeted other species, they provide satisfactory spatiotemporal coverage
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Received 13 January 2003; Accepted after revision 3 February 2004
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We need to build on those and try and test new things
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Besides conducting interviews with seniors and other site users, Medco worked with Cooper Interaction Design of Palo Alto, Calif
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